Journal: Proceedings of SPIE--the International Society for Optical Engineering
Article Title: Comparative investigation of stimulus-evoked rod outer segment movement and retinal electrophysiological activity
doi: 10.1117/12.2249548
Figure Lengend Snippet: (A) Schematic diagram of the experimental setup. During the experiment, the frog retina was continuously illuminated by a NIR light source in a transmission mode for image acquisition and superfused with physiological medium. Visible light generated by a fiber-coupled light emitting diode (LED) was collimated and coupled into the light path of the microscope to work as a stimulus. ERG recording was implemented by placing two differential electrodes on the top and bottom of the retina, respectively. (B) The retina was placed in a glass chamber with ROSs facing upward and was obliquely (∼20°) stimulated by a visible light beam from the top. The direction of ROS movement was defined by the coordinate axes shown in the middle.
Article Snippet: The acquisition of ROS movements was achieved by imaging the retina with a modified NIR light microscope (BX531WI, Olympus, Japan) and a CCD camera (pixel size: 7.4 μm × 7.4 μm; Pike, Allied Vision, Exton, PA).
Techniques: Transmission Assay, Generated, Microscopy